Kvasny Prum. 2008; 54(11-12): 338-343 | DOI: 10.18832/kp2008019

Strictly anaerobic bacteria in beer and in breweries.Peer-reviewed article

Dagmar MATOULKOVÁ
Výzkumný ústav pivovarský a sladařský, a. s., Lípová 15, 120 44 Praha 2

The content of oxygen is one of the main factors affecting microbiological stability of beer. The current trend in the development of bottling technologies therefore accentuates lowering of oxygen tension in finished beer to a minimum. Beer thus becomes a medium in which strictly anaerobic bacteria that require for their growth and proliferation very low or zero oxygen level can thrive. Strictly anaerobic bacteria capable of spoiling beer or contaminating brewer's yeast belong to the genera Pectinatus, Megasphaera, Zymophilus and Selenomonas. The increasing frequency of beer spoilage cases is also promoted by increasing production of beers that have a higher propensity to microbial spoilage - nonpasteurized, nonalcoholic, low-alcoholic and low hopped beers. A survey is presented here of basic morphological, biochemical and physiological properties of this group of bacteria.The content of oxygen is one of the main factors affecting microbiological stability of beer. The current trend in the development of bottling technologies therefore accentuates lowering of oxygen tension in finished beer to a minimum. Beer thus becomes a medium in which strictly anaerobic bacteria that require for their growth and proliferation very low or zero oxygen level can thrive. Strictly anaerobic bacteria capable of spoiling beer or contaminating brewer's yeast belong to the genera Pectinatus, Megasphaera, Zymophilus and Selenomonas. The increasing frequency of beer spoilage cases is also promoted by increasing production of beers that have a higher propensity to microbial spoilage - nonpasteurized, nonalcoholic, low-alcoholic and low hopped beers. A survey is presented here of basic morphological, biochemical and physiological properties of this group of bacteria.

Keywords: strictly anaerobic bacteria, Pectinatus, Zymophilus, Selenomonas, Megasphaera, beer spoilage, yeast contamination

Published: November 1, 2008 

References

  1. Sakamoto, K., Konings, W. N.: Beer spoilage bacteria and hop resistance. Int. J. Food Microbiol. 89, 2003, 105-124. Go to original source...
  2. Juvonen, R., Koivula, T., Haikara, A.: Group-specific PCR-RFLP and real-time PCR methods for detection and tentative discrimination of strictly anaerobic beer-spoilage bacteria of the class Clostridia. Int. J. Food Microbiol. 128, 2008, 162-169. Go to original source...
  3. Sedláček, I.: Taxonomie prokaryot. 1. vydání, Masarykova univerzita, Brno, 2007.
  4. Back, W.: Secondary contaminations in the filling area. Brauwelt Int. 4, 1994, 326-334.
  5. Dragone, G., Mussatto, S. I., Nogueira, A. D., de Almeida, e Silva J. B.: Review: Beer production: Spoilage microorganisms and detection methods. Braz. J. Food Technol. 10, 2007, 240-251.
  6. Schleifer, K. H., Leuteritz, M., Weiss, N., Ludwig, W., Kirchhof, G., Sedel-Rüfer, H.: Taxonomic study of anaerobic, gram-negative, rod-shaped bacteria from breweries:Emended description of Pectinatus cerevisiiphilus and description of Pectinatus frisingensis sp. nov., Selenomonas lacticifex sp. nov., Zymophilus rafinosivorans gen. nov., sp. nov., and Zymophilus paucivorans sp. nov. Int. J. Syst. Bacteriol. 40, 1990, 19-27. Go to original source...
  7. Gonzales, J. M., Jurado, V., Laiz, L., Zimmermann, J., Hermosin, B., Saiz-Jimenez, C.: Pectinatus portalensis nov.sp., a relatively fast-growing, coccoidal, novel Pectinatus species isolated from a wastewater treatment plant. Antonie Leeuwen. 86, 2004, 241-248. Go to original source...
  8. Juvonen, R., Suihko, M. L.: Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus. Int. J. Syst.Evolution. Microbiol. 56, 2006, 695-702. Go to original source...
  9. Lee, S. Y., Mabee, M. S., Jangaard, N. O., Horiuchi, E. K.: Pectinatus, a new genus of bacteria capable of growth in hopped beer. J. Inst. Brew. 86, 1980, 28-30. Go to original source...
  10. Haikara, A., Penttilä, L., Enari, T. M., Lounatmaa, K.: Microbiological, biochemical, and electron microscopic characterization of a Pectinatus strain. Appl. Environ. Microbiol. 41, 1981b, 511-517. Go to original source...
  11. Back, W.: Nachweiss und Identifizierung gramnegativer bierschädlicher Bakterien. Brauwissenschaft 34, 1981, 197-204.
  12. Chelack, B. J., Ingledew, W. M.: Anaerobic gram-negative bacteria in brewing - A review. J. Am. Soc. Brew. Chem. 45, 1987, 123-127. Go to original source...
  13. Haikara, A., Enari, T.-M., Lounatmaa, K.: The genus Pectinatus, a new group of anaerobic beer spoilage bacteria. Proc. EBC, Copenhagen, 1981, 229-240.
  14. Helander, I. M., Haikara, A., Sadovskaya, I., Vinogradov, E., Salkinoja-Salonen, M.S.: Lipopolysaccharides of anaerobic beer spoilage bacteria of the genus Pectinatus - lipopolysaccharides of a gram-positive genus. FEMS Microbiol. Rev. 28, 2004, 543-552. Go to original source...
  15. Chaban, B., Deneer, H., Dowgiert, T., Hymers, J., Ziola, B.: The flagellin gene and protein from the brewing spoilage bacteria Pectinatus cerevisiiphilus and Pectinatus frisingensis. Can. J. Microbiol. 51, 2005, 863-874. Go to original source...
  16. Membré, J.-M., Tholozan, J.-L.: Modeling growth and off-flavours production of spoiled beer bacteria, Pectinatus frisingensis. J. Appl. Bacteriol. 77, 1994, 456-460. Go to original source...
  17. Haikara, A.: Immunological characterization of Pectinatus cerevisiiphilus strains. Appl. Environ. Microbiol. 46, 1983, 1054-1058. Go to original source...
  18. Back, W., Breu, S., Weigand, C.: Infektionsursachen im Jahre 1987. Brauwelt 138, 1988, 1358-1362.
  19. Watier, D., Leguerinel, I., Hornez, J. P., Chowdhury, I., Dubourguier, H. C.: Heat resistance of Pectinatus sp., a beer spoilage anaerobic bacterium. J. Appl. Bacteriol. 78, 1995, 164-168. Go to original source...
  20. Chihib, N.-E., Tholozan, J.-L.: Effect of rapid cooling and acidic pH on cellular homeostasis of Pectinatus frisingensis, a strictly anaerobic beer-spoilage bacterium. Int. J. Food Microbiol. 48, 1999, 191-202. Go to original source...
  21. Haikara, A.: Detection of Pectinatus contaminants in beer. J. Am. Soc. Brew. Chem. 43, 1985, 43-46. Go to original source...
  22. Back, W., Weiss, N., Seidel, H.: Isolierung und systematische Zuordnung bierschädlicher gramnegativer Bakterien.II.: Gramnegative anaerobe Stäbchen (Anhang: Aus Bier isolierte gramnegative fakultativ anaerobe Stäbchen). Brauwissenschaft 32, 1979, 233-238.
  23. Takahashi, N.: Presumed Pectinatus strain isolated from Japanese beer. Bull. Brew. Science 28, 1983, 11-14.
  24. Chowdhury, I., Watier, D., Hornez, J.-P.: Variability in survival of Pectinatus cerevisiiphilus, strictly anaerobic bacteria, under different oxygen conditions. Anaerobe 1, 1995, 151-156. Go to original source...
  25. Bendová, O., Kurzová, V.: Gramnegativní bakterie kontaminující pivo. Kvasny Prum. 27, 1981, 268-271. Go to original source...
  26. Watier, D., Leguerinel, I., Hornez, J. P., Dubourguier, H. C.: Influence of physicochemical parameters on growth kinetics of Pectinatus cerevisiiphilus and Megasphaera cerevisiae. Sci. Alim. 13, 1993, 297-304.
  27. Tholozan, J.-L., Grivet, J.-P., Vallet, C.: Metabolic pathway to propionate of Pectinatus frisingensis, a strictly anaerobic beer-spoilage bacterium. Arch. Microbiol. 162, 1994, 401-408. Go to original source...
  28. Tholozan, J.-L., Membré, J.-M., Grivet, J.P.: Physiology and development of Pectinatus cerevisiiphilus and Pectinatus frisingensis, two strict anaerobic beer spoilage bacteria. Int. J. Food Microbiol. 35, 1997, 29-39. Go to original source...
  29. Vaughan, A., O'Sullivan, T., van Sinderen, D.: Enhancing the microbiological stability of malt and beer - A review. J. Inst. Brew. 111, 2005, 355-371. Go to original source...
  30. Suiker, I., O'Sullivan, T., Vaughan, A.: Diversity analysis of beer spoiling gram-negative isolates using PCR fingerprinting and computer-assisted analysis. Prco. EBC, Venice, 2007, 1098-1105.
  31. Seidel-Rüfer, H.: Pectinatus und andere morphologisch ähnliche gram-negative, anaerobe Stäbchen aus dem Brauereibereich. Mschr. Brauwissenschaft 43, 1990, 101-105.
  32. Henriksson, E., Haikara, A.: Airborne microorganisms in the brewery filling area and their effect on microbiological stability of beer. Mschr. Brauwissenschaft 44, 1991, 4-8.
  33. Hakalehto, E., Finne, J.: Identification by immunoblot analysis of major antigenic determinants of the anaerobic beer spoilage bacterium genus Pectinatus. FEMS Microbiol. Lett. 67, 1990, 307-312. Go to original source...
  34. Motoyama, Y., Ogata, T.: 16S-23S rDNA spacer of Pectinatus, Selenomonas and Zymophilus reveal new phylogenetic relationships between these genera. Int. J. Syst. Evolution. Microbiol. 50, 2000, 883-886. Go to original source...
  35. Jespersen, L., Jakobsen, M.: Specific spoilage organisms in breweries and laboratory media for their detection. Int. J. Food Microbiol. 33, 1996, 139-155. Go to original source...
  36. Kingsley, V.V., Hoeniger, J. F. M.: Growth, structure and classification of Selenomonas. Bacteriol. Rev. 37, 1973, 479-521. Go to original source...
  37. Haikara, A.: Invasion of anaerobic bacteria into pitching yeast. Proc. EBC, Zürich, 1989, 537-544.
  38. Marchandin, H., E. Jumas-Bilak, B. Gay, C. Teyssier, H. Jean-Pierre, M. S. de Buochberg, C. Carriere, and J. P. Carlier.: Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp. nov. Int. J. Syst. Evol. Microbiol. 53, 2003, 547-553. Go to original source...
  39. Weiss, N., Seidel, H., Back, W.: Isolierung und systematische Zuordnung bierschädlicher gramnegativer Bakterien. I.: Gramnegative strikt anaerobe Kokken. Brauwissenschaft 32, 1979, 189-194.
  40. Ziola, B., Gee, L., Berg, N. N., Lee, S. Y.: Serogroups of the beer spoilage bacterium Megasphaera cerevisiae correlate with the molecular weight of the major EDTA-extractable surface protein. J. Microbiol. 46, 2000, 95-100. Go to original source...
  41. Suihko, M. L., Haikara, A.: Characterization of Pectinatus and Megasphaera strains by automated ribotyping. J. Inst. Brew. 107, 2001, 175-184. Go to original source...
  42. Haikara, A., Lounatmaa, K.: Characterization of Megasphaera sp., a new anaerobic beer spoilage coccus. Proc. EBC, Madrid, 1987, 473-480.
  43. Watier, D., Chodhury, I., Leguerinel, I., Hornez, J. P.: Survival of Megasphaera cerevisiae heated in laboratory media, wort and beer. Food Microbiol. 13, 1996, 205-212. Go to original source...
  44. Šavel, J.: Mikrobiologická kontrola v pivovarech. 1. vydání, SNTL, Praha, 1980.
  45. Haikara, A.: Detection of anaerobic, gram-negative bacteria in beer. Mschr. Brauwissenschaft 38, 1985, 239-243.

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