Kvasny Prum. 2013; 59(10-11): 313-316 | DOI: 10.18832/kp2013033

Sequencing the genome of bottom brewer's yeast.Peer-reviewed article

Pavel DOSTÁLEK, Jan KVASNIČKA, Rudolf CEJNAR, Jaroslav VOHANKA, Martin MOKREJŠ, Jaroslava HÁJKOVÁ, Petra BOBČÍKOVÁ, Tomáš BRÁNYIK, Karel MELZOCH, Karel SEDLÁŘ
1 Ústav biotechnologie, Vysoká škola chemicko-technologická v Praze, Technická 5, Praha 6, 166 28
2 Trombotické centrum a Centrální hematologické laboratoře, Ústav klinické biochemie a laboratorní diagnostiky, Všeobecná fakultní nemocnice a 1. Lékařská fakulta UK Praha
3 ROCHE s.r.o., Karlovo náměstí 17, Praha 2
4 Ústav biomedicínského inženýrství, Fakulta elektrotechniky a komunikačních technologií, Vysoké učení technické v Brně, Technická 12, 616 00 Brno

Saccharomyces pastorianus CCY48-91 is a strain of bottom-fermenting brewing yeast most commonly used in Czech brewing. The main goal of this study is to sequence its genome and make this assembly publicly available. Because there are not many industrial yeast strains that have been sequenced, this data could be of value to other researchers.Saccharomyces pastorianus CCY48-91 is a strain of bottom-fermenting brewing yeast most commonly used in Czech brewing. The main goal of this study is to sequence its genome and make this assembly publicly available. Because there are not many industrial yeast strains that have been sequenced, this data could be of value to other researchers.

Keywords: genome, yeast, Saccharomyces pastorianus

Received: August 15, 2013; Accepted: October 1, 2013; Published: October 1, 2013 

References

  1. Bartlett, J. M. S.; Stirling, D., 2003: A Short History of the Polymerase Chain Reaction. PCR Protocols 226: 3-6. Go to original source...
  2. Borsting, C., Hummel, R., Schultz, E.R., Rose, T.M., Pedersen, M.B., Knudsen, J., Kristiansen, K., 1997: Saccharomyces carlsbergensis contains two functional genes encoding the acyl-CoA binding protein, one similar to the ACB1 gene from S. cerevisiae and one identical to the ACB1 gene from S. monacensis. Yeast 13: 1409-1421. Go to original source...
  3. Casaregola, S., Nguyen, H.-V., Lapathitis, G., Kotyk, A., Gaillardin, C., 2001: Analysis of the constitution of the beer yeast genome by PCR, sequencing and subtelomeric sequence hybridization. Int. J. Syst. Evol. Microbiol. 51: 1607-1618. Go to original source...
  4. Dunn, B., Sherlock, G., 2008: Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianus. Genome Res. 18: 1610-1623. Go to original source...
  5. Fernandez-Espinar, M.T., Barrio, E., Querol, A., 2003: Analysis of the genetic variability in the species of the Saccharomyces sensu stricto complex. Yeast 20: 1213-1226. Go to original source...
  6. Fujii, T., Yoshimoto, H., Nagasawa, N., Bogaki, T., Tamai, Y., Hamachi, M., 1996: Nucleotide sequences of alcohol acetyltransferase genes from lager brewing yeast, Saccharomyces carlsbergensis. Yeast 12: 593-598. Go to original source...
  7. Gonzalez, S.S., Barrio, E., Querol, A., 2008: Molecular characterization of new natural hybrids of Saccharomyces cerevisiae and S. kudriavzevii in brewing. Appl. Environ. Microbiol. 74: 2314-2320. Go to original source...
  8. Hansen, J., Cherest, H., Kielland-Brandt, M.C., 1994: Two divergent MET10 genes, one from Saccharomyces cerevisiae and one from Saccharomyces carlsbergensis, encode the α subunit of sulfite reductase and specify potential binding sites for FAD and NADPH. J. Bacteriol. 176: 6050-6058. Go to original source...
  9. Hansen, J., Kielland-Brandt, M.C., 1994: Saccharomyces carlsbergensis contains two functional MET2 alleles similar to homologs from S. cerevisiae and S. monacensis. Gene 140: 33-40. Go to original source...
  10. Joubert, R., Brignon, P., Lehmann, C., Monribot, C., Gendre, F., Boucherie, H., 2000: Two-dimensional gel analysis of the proteome of lager brewing yeasts. Yeast 16: 511-522. Go to original source...
  11. Kleppe, K., Ohtsuka, E., Kleppe, R., Molineux, I., Khorana, H.G., 1971: Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA's as catalyzed by DNA polymerases. J. Mol. Biol. 56(2): 341-361. Go to original source...
  12. Kodama, Y., Omura, F., Ashikari, T., 2001: Isolation and characterization of a gene specific to lager brewing yeast that encodes a branched-chain amino acid permease. Appl. Environ. Microbiol. 67: 3455-3462. Go to original source...
  13. Martini, A.V., Martini, A., 1987: Three newly delimited species of Saccharomyces sensu stricto. Antonie Van Leeuwenhoek 53: 77-84. Go to original source...
  14. Nakao, Y., Kanamori, T., Itoh, T., Kodama, Y., Rainieri, S., Nakamura, N., Shimonaga, T., Hattori, M., Ashikari, T., 2009: Genome sequence of the lager brewing yeast, an interspecies hybrid. DNA Res. 16: 115-129. Go to original source...
  15. Querol, A., Bond, U., 2009: The complex and dynamic genomes of industrial yeasts. FEMS Microbiol. Lett. 293: 1-10. Go to original source...
  16. Rainieri, S., Kodama, Y., Kaneko, Y., Mikata, K., Nakao, Y., Ashikari, T., 2006: Pure and mixed genetic lines of Saccharomyces bayanus and Saccharomyces pastorianus and their contribution to the lager brewing strain genome. Appl. Environ. Microbiol. 72: 3968-3974. Go to original source...
  17. Sanger, F., Coulson, A.R., 1975: A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 94 (3): 441-448. Go to original source...
  18. Sanger, F., Nicklen, S., Coulson, A.R., 1977: DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74 (12): 5463-5467. Go to original source...
  19. Saerens, S.M.G., Duong, C.T., Nevoigt, E., 2010: Genetic improvement of brewer's yeast: current state, perspectives and limits. Appl. Microbiol. Biotechnol. 86: 1195-1212. Go to original source...
  20. Shendure, J., Porreca, G.J., Reppas, N.B., Lin, X., McCutcheon, J.P., Rosenbaum, A.M., Wang, D., Zhang Kun, Mitra, R.D., Church, G.M., 2005: Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome. Science 309 (5741): 1728-1732. Go to original source...
  21. Tamai, Y., Momma, T., Yoshimoto, H., Kaneko, Y., 1998: Co-existence of two types of chromosome in the bottom fermenting yeast, Saccharomyces pastorianus. Yeast 14: 923-933. Go to original source...
  22. Tamai, Y., Tanaka, K., Umemoto, N., Tomizuka, K., Kaneko, Y., 2000: Diversity of the HO gene encoding an endonuclease for mating-type conversion in the bottom fermenting yeast Saccharomyces pastorianus. Yeast 16: 1335-1343. Go to original source...
  23. Wicker, T., Schlagenhauf, E., Graner, A., Close T.J., Keller B., Stein N., 2006: 454 sequencing put to the test using the complex genome of barley, BMC Genomics. 7: 275. Go to original source...
  24. Yamagishi, H., Ogata, T., 1999: Chromosomal structures of bottom fermenting yeasts. Syst. Appl. Microbiol. 22: 341-353. Go to original source...