Kvasny Prum. 1976; 22(5): 111-114 | DOI: 10.18832/kp1976018

Mass balance in batch cultivation of Candida utilis with synthetic ethanol.Peer-reviewed article

M. RUT, L. ADÁMEK, F. ŠTROS, K. PECKA

During synthesis of yeast biomass in batsch culture with ethanol the efficiency of substrate utilization ethanol the efficiency of substrate utilization varies with phases of the process. In logarithmic phase the specific oxigen demand and respiration quotient are rising whereas the yields drop. The cultivation process is accompanied by formation of an undesirable intermediate oxidation product, i.e. acetic acid, Which  above certain limits affects unfavorably some characteristics taken into calculations of mass balance. The authors have derived stoichiometric equations for the initial phases of the process, when the oxygen demand is low and substrate is utilized very efficiently as well as for final ones, when accumulated acetic acid affects the biomass synthesis and increases the oxygen demand. The total balance of process is given by the sun covering all phases. From the analysis of results the authors calculate a mass balance for well balanced process without acetic acid accumulation. Such a process offers yields which for given strain, raw materials and technology are maximum which can be obtained.
(In Czech, English summary only)

Keywords: Candida utilis, mass bilancy, ethanol

Published: May 1, 1976