Kvasny Prum. 2016; 62(7-8): 198-205 | DOI: 10.18832/kp2016024

Non-Saccharomyces Yeasts and Their Importance in the Brewing Industry Part I -Brettanomyces (Dekkera)Peer-reviewed article

Tatiana KOCHLÁŇOVÁ, David KIJ, Jana KOPECKÁ, Petra KUBIZNIAKOVÁ, Dagmar MATOULKOVÁ
1 Ústav experimentální biologie, Přírodovědecká fakulta, Masarykova Univerzita / Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, e-mail: 376098@mail.muni.cz, 223187@mail.muni.cz
2 Mikrobiologické oddělení, Výzkumný ústav pivovarský a sladařský, a.s., / Department of Microbiology, Research Institute of Brewing and Malting, PLC, Lípová 15, 120 44 Prague e-mail: kubizniakova@beerresearch.cz, matoulkova@beerresearch.cz

In the history of beer production, yeasts of genera other than Saccharomyces occurred as part of a spontaneous fermentation. They are now mostly regarded as contaminants producing undesirable sensorially active substances and CO2. Exceptions are special beer styles (e.g. lambic and gueuze), in which the metabolic activity of non-Saccharomyces yeasts (in particular genera Brettanomyces and Dekkeraa) is a key element. The article gives the characteristics and taxonomy of Brettanomyces and Dekkera yeast and describes the basic microbiological aspects of brewing lambic and gueuze. In der Geschichte des Bieres wurden auch die anderen Hefestämme außer Stamm Saccharomyces betrachtet, weil die einen Teil der Spontangärungshefe darrstellen. Zur Zeit werden als eine Kontamination betrachten, weil die eine unerwünschte sensorische Aktivstoffe und CO2 verursachen. Die Ausnahme tun die spezielle Biertypen (z. B. Bier Lambic und Gueuze) wo die metabolische Tätigkeit von Nicht-Saccharomyces Hefe (insbesondere die Stämme Brettanomyces und Dekkera) stellt ein Schlüsselelement dar. Im Artikel werden die Charakteristik und eine taxonomische Einordnung von Stämmen Brettanomyces und Dekkera angeführt und grundmikrobiologische Aspekte der Lambik- und Gueuze Bierherstellung beschrieben.

Keywords: Brettanomyces, Dekkera, lambic, gueuze, non-Saccharomyces, spontaneous fermentation

Received: May 28, 2016; Accepted: June 22, 2016; Published: August 10, 2016 

References

  1. Barnett, A. J, Entian K.D., 2005: A history of research on yeasts 9: Regulation of sugar metabolism. Yeast 22: 835- 894. Go to original source...
  2. Blomqvist, J., Eberhard, T., Schnürer, J., Passoth, V., 2010: Fermentation characteristics of Dekkera bruxellensis strains. Appl. Microbiol. Biotechnol. 87: 1487-97. Go to original source...
  3. Bokulich, N.A., Bamforth, C.W., Mills, D.A., 2012: Brewhouse-resident microbiota are responsible for multi-stage fermentation of American Coolship Ale. PLoS ONE 7: e35507. DOI: 10.1371/journal.pone.0035507. Go to original source...
  4. Carrascosa, J.M., Viguera, M.D., Nuez de Castro, I., Scheffers, W.A., 1981: Metabolism of acetaldehyde and Custer effect in the yeast Brettanomyces abstinens. Antonie Van Leeuwenhoek 47: 209-215. Go to original source...
  5. Clemente-Jiménez, J.M., Mingorance-Cazorla, L., Martínez-Rodríguez, S., Las Heras-Vázquez, F.J., Rodríguez-Vico, F., 2004: Molecular characterization and oenological properties of wine yeasts isolated during spontaneous fermentation of six varieties of grape must. Food Microbiol. 21: 149-155. Go to original source...
  6. Conterno, L., Aprea, E., Franceschi, P., Viola, R., Vrhovsek, U., 2013: Overview of Dekkera bruxellensis behaviour in an ethanol-rich environment using untargeted and targeted metabolomic approaches. Food. Res. Int. 51: 670-678. Go to original source...
  7. Daenen, L., Sterckx, F., Delvaux, F.R., Verachtert, H., Derdelinckx, G., 2008: Evaluation of the glycoside hydrolase activity of a Brettanomyces strain on glycosides from sour cherry (Prunus cerasus L.) used in the production of special fruit beers. FEMS Yeast Res. 8:1103-1114. Go to original source...
  8. De Deken, R.H., 1966: The Crabtree effect: a regulatory system in yeast. J. Gen. Microbiol. 44: 149-156. Go to original source...
  9. De Keersmaecker, J., 1996: The mystery of lambic beer. Sci. Am. 275: 74-81. Go to original source...
  10. Dujon, B., 2010: Yeast evolutionary genomics. Nat. Rev Genet. 11: 512-524. Go to original source...
  11. Gaunt, D.M., Degn, H., Lloyd, D., 1988: The influence of oxygen and organic hydrogen acceptors on glycolytic carbon dioxide production in Brettanomyces anomalus. Yeast 4: 249-255. Go to original source...
  12. Guinard, J., 1990: Lambic, Brewers Publications, Boulder, Colorado, USA, ISBN 0937381225.
  13. Hawksworth, D.L., 2012: Managing and coping with names of pleomorphic fungi in a period of transition. Mycosphere, 3: 52-64; IMA Fungus 3: 15-24. Go to original source...
  14. Johnson, A.E., 2013a: Biotechnology of non-Saccharomyces yeasts-the ascomycetes. Appl. Microbiol. Biotechnol. 97: 503-517, doi: 10.1007/s00253-012-4497-y. Go to original source...
  15. Johnson, A.E., 2013b: Biotechnology of non-Saccharomyces yeasts-the basidiomycetes. Appl. Microbiol. Biotechnol. 97: 7563-7577, doi: 10.1007/s00253-013-5046-z. Go to original source...
  16. Kumara, H.M.C.S., De Cort, S., Verachtert, H., 1993: Localization and characterization of alpha-glucosidase activity in Brettanomyces lambicus. Appl. Environ. Microbiol. 59: 2352-2358. Go to original source...
  17. Kurtzman, C.P., Fell, J.W., Boekhout, T., 2011: The Yeast, a taxonomic study, 5th edition, Elsevier, Burlington, USA, 2384 s. ISBN 978-0-444-52149-1.
  18. Leite, F.C.B., Basso, T.O., WdB, P., Gombert, A.K., Simões, D.A., Morais, M.A., 2013: Quantitative aerobic physiology of the yeastDekkera bruxellensis, a major contaminant in bioethanol production plants. FEMS Yeast Res. 13: 34-43. Go to original source...
  19. Němec, M., Matoulková, D., 2015: Základy obecné mikrobiologie, 1. vyd., Masarykova univerzita, Brno, 256 s. ISBN 978-80-210-7923-6.
  20. Oelofse, A., Pretorius, I.S., du Toit, M., 2008: Significance of Brettanomyces and Dekkera during winemaking: a synoptic review. S. Afr. J. Enol. Vitic. 29: 128-144. Go to original source...
  21. Pretorius, I.S., Van Der Westhuizen, T.J., Augustyn, O.P.H., 1999: Yeast biodiversity in vineyards and wineries and its importance to the South African wine industry. S. Af. Enol. Vitic. 20(2): 61-74. Go to original source...
  22. Romano, A., Perello, M.C., de Revel, G., Lonvaud-Funel, A., 2008: Growth and volatile compound production by Brettanomyces/Dekkera bruxellensis in red wine. J. Appl. Microbiol. 104: 1577-1585. Go to original source...
  23. Scheffers, W.A., Nanninga, G.L., 1977: The anaerobic inhibition of fermentation (Custers effect) in Brettanomyces. EUCHEM Conference on Metabolic Reactions in the Yeast Cell in Anaerobic and Aerobic Conditions (Helsinki, Finland) p. 53-55.
  24. Souciet, J.L., Dujon, B., Gaillardin, C., et al., 2009: Comparative genomics of protoploid Saccharomycetaceae. Cold Spring Harbor Laboratory Press, 19: 1696-1709, doi: 0.1101/gr.091546.109. Go to original source...
  25. Spaepen, M., Van Oevelen, D., Verachtert, H., 1978: Fatty acids and esters produced during the spontaneous fermentation of lambic and gueuze. J. Inst. Brew. 84: 278-282. Go to original source...
  26. Spitaels, F., Wieme, A.D., Janssens, M., Aerts, M., Daniel, H.M., Van Landschoot, A., De Vuyst, L., Vandamme, P., 2014: The microbial diversity of traditional spontaneously fermented lambic beer. PLoS ONE, 9, e95384. DOI: 10.1371/journal.pone.0095384. Go to original source...
  27. Steensels, J., Daenen, L., Malcorps, P., Derdelinckx, G., Verachtert, H., Verstrepen, K.J., 2015: Brettanomyces yeasts - From spoilage organisms to valuable contributors to industrial fermentations. Int. J. Food Microbiol. 206: 24-38. Go to original source...
  28. Steensels, J., Verstrepen, K.J., 2014: Taming wild yeast: potential of conventional and nonconventional yeasts in industrial fermentations. Annu. Rev. Microbiol. 68: 61-80, doi: 10.1146/annurev-micro-091213-113025. Go to original source...
  29. Sturm, M., Arroyo-López, F., Garrido-Fernández, A., Querol, A., Mercado, L., Ramirez, M., Combina, M., 2014: Probabilistic model for the spoilage wine yeast Dekkera bruxellensis as a function of pH, ethanol and free SO2 using time as a dummy variable. Int. J. Food Microbiol. 170: 83-90. Go to original source...
  30. Vanderhaegen, B., Neven, H., Coghe, S., Verstrepen, K.J., Derdelinckx, G., Verachtert, H., 2003: Bioflavoring and beer refermentation. Appl. Microbiol. Biotechnol. 62: 140-150. Go to original source...
  31. Van der Walt, J.P., 1984: Dekkera. In: Kreger-van Rij, N.J.W. (Ed.), The Yeasts: A Taxonomic Study, 3th edition Elsevier Science, Amsterdam, pp. 146-150.
  32. Van Oevelen, D., Delescaille, F., Verachtert, H., 1976: Synthesis of aroma components during spontaneous fermentation of lambic and gueueze. J. Inst. Brew. 82: 322-326. Go to original source...
  33. Van Oevelen, D., Spaepen, M., Timmermans, P., Verachtert, H., 1977: Microbiological aspects of spontaneous wort fermentation in the production of lambic and gueuze. J. Inst. Brew. 83: 356-360. Go to original source...
  34. Verachtert, H., Dawoud, E., Kumara, H., 1989: Interactions between Enterobacteriaceae and Saccharomyces cerevisiae during wort fermentation. Yeast 5: 67-72.
  35. Verachtert, H., Iserentant, D., 1995: Properties of Belgian acid beers and their microflora. Part I. The production of gueuze and related refreshing acid beer. Cerevisia, Belgian J. Brew. Biotechnol. 20: 37-41.
  36. Verstrepen, K.J., Derdelinckx, G., Dufour, J.P., Winderickx, J., Thevelein, J.M., Pretorius, I.S., Delvaux, F.R., 2003: Flavor-active esters: adding fruitiness to beer. J. Biosci. Bioeng. 96: 110-118. Go to original source...
  37. Zarnkow, M., Faltermaier, A., Back, W., Gastl, M., Arendt, E.K., 2010: Evaluation of different yeast strains on the quality of beer produced from malted proso millet (Panicum miliaceum L.). Eur. Food Res. Technol. 231: 287-295. Go to original source...