Kvasny Prum. 2002; 48(3): 58-61 | DOI: 10.18832/kp2002005

Application of Gel-Permeation Chromatography for Determination of Organic Pollutants in Brewing Barley.Peer-reviewed article

T. HORÁK, M. JURKOVÁ, J. ČULÍK, P. ČEJKA, V. KELLNER

A  method   was  developed  that   enables,  within  one extraction  step,  to  determine  nine  polycyclic  aromatic hydrocarbons    (PAH)   -    benzo(a)anthracene,   chrysene, benzo(b)fluoranthene,  benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene,    indeno(1,2,3-cd)pyrene,dibenzo(a, i)pyrene,   dibenzo(a,h)pyrene   -   and   seven  indicating congeners of polychlorinated biphenyls  (PCB) - congeners of No. 28, 52, 101, 118, 138, 153, 180 - in vegetable matrixes, mainly in  barley and malt.  The assay process  includes the description of  extraction and purification  of the acquired extract by means of the gel-permeation chromatography method with   the  use   of  gel   on  the   basis  of  polymer  of styrenedivinylbenzene   (Bio-Beads  SX-3).In   this  way  an extract   is   obtained   of   sufficient   purity  for  the determination of  both PAH and  PCB. The final  assay of PAH was  carried  out  by  the  method  of high-efficient liquid chromatography and  the assay of  PCB by gas  chromatography using the electron capture detector. All analytes can be set in microgram amounts with a relative authoritative deviation < 25 %.     The study contains characteristics of the method for all stipulated substances and speciment chromatograms.A  method   was  developed  that   enables,  within  one extraction  step,  to  determine  nine  polycyclic  aromatic hydrocarbons    (PAH)   -    benzo(a)anthracene,   chrysene, benzo(b)fluoranthene,  benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene,    indeno(1,2,3-cd)pyrene,dibenzo(a, i)pyrene,   dibenzo(a,h)pyrene   -   and   seven  indicating congeners of polychlorinated biphenyls  (PCB) - congeners of No. 28, 52, 101, 118, 138, 153, 180 - in vegetable matrixes, mainly in  barley and malt.  The assay process  includes the description of  extraction and purification  of the acquired extract by means of the gel-permeation chromatography method with   the  use   of  gel   on  the   basis  of  polymer  of styrenedivinylbenzene   (Bio-Beads  SX-3).In   this  way  an extract   is   obtained   of   sufficient   purity  for  the determination of  both PAH and  PCB. The final  assay of PAH was  carried  out  by  the  method  of high-efficient liquid chromatography and  the assay of  PCB by gas  chromatography using the electron capture detector. All analytes can be set in microgram amounts with a relative authoritative deviation < 25 %.     The study contains characteristics of the method for all stipulated substances and speciment chromatograms.

Keywords: PAH, PCB, malting barley, beer, gel permeation chromatography

Published: March 1, 2002